Transforming growth factor beta
轉化生長因子β(TGF-β)是一種多功能的細胞因子,屬于轉化生長因子超家族,包括三種不同的哺乳動物異構體(TGF-β 1至3,HGNC符號TGFB1、TGFB2、TGFB3)和許多其他信號蛋白。TGFB蛋白由所有白血球系產生。被激活的TGF-β與其他因子復合形成絲氨酸/蘇氨酸激酶復合物,與TGF-β受體結合。TGF-β受體由1型和2型受體亞單位組成。
可檢測樣本中小鼠的TGF-β,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿、細胞培養上清中加入3個不同濃度水平的小鼠TGF-β,計算回收率
分別在選取的4份健康小鼠血清、血漿、細胞培養上清中加入高濃度小鼠TGF-β,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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