Interleukin 12
白細胞介素12(IL-12)是一種白細胞介素,由樹突狀細胞、巨噬細胞、中性粒細胞和B淋巴細胞(NC-37)在抗原刺激下自然產生。IL-12屬于白細胞介素-12家族,在自然殺傷細胞和T淋巴細胞的活動中起重要作用。IL-12還具有抗血管生成活性,這意味著它可以阻止新血管的形成。IL-12的是由二硫鍵相連異源二聚體,70 kDa (p70)的 異二聚體糖蛋白由40 kDa (p40)和35 kDa (p35)兩個亞基組成。
可檢測樣本中小鼠的IL-12,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿、細胞培養上清中加入3個不同濃度水平的小鼠IL-12,計算回收率
分別在選取的4份健康小鼠血清、血漿、細胞培養上清中加入高濃度小鼠IL-12,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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