NOS2A;NOS2; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2
誘導型NO合酶(iNOS)是由氨基酸l-arginine生成一氧化氮(NO)的三種關鍵酶之一。INOS衍生的NO在許多生理(如血壓調節、傷口修復和宿主防御機制)和病理生理(炎癥、感染、腫瘤性疾病、肝硬化、糖尿病)條件下發揮著重要作用。然而,INOS在腫瘤發展過程中的作用是非常復雜的,如蛻變、血管生成和轉移,是由INOS調節的。另一方面,來自巨噬細胞的NO對腫瘤細胞有潛在的細胞毒性/細胞抑制作用。因此,對INOS活動的治療干預具有相當大的意義,特別是在腫瘤中,轉移活動、宿主防御機制和分化水平與INOS的表達相關。
可檢測樣本中小鼠的iNOS,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠組織勻漿、細胞培養上清中加入3個不同濃度水平的小鼠iNOS,計算回收率
分別在選取的4份健康小鼠組織勻漿、細胞培養上清中加入高濃度小鼠iNOS,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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