S-IgA
分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)是20世紀60年代初在外分泌液中發現的一種IgA抗體,主要存在于乳汁、胃腸液、呼吸道分泌液等外分泌液中。SIgA分子是由2個IgA單體(每個單體含2條輕鏈和2條重鏈)、1條J鏈和1條分泌片(secretory component,SC,為多聚免疫球蛋白受體的胞外裂解片段)構成的異源十聚體,為了與血清IgA單體相區別而被命名為SIgA。SIgA是外分泌液中存在的一種主要抗體,是呼吸道、消化道、泌尿生殖道等抵御病原體及有害物質的第一道免疫防線,是機體黏膜免疫最重要的抗體。
可檢測樣本中小鼠的sIgA,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿、細胞培養上清中加入3個不同濃度水平的小鼠sIgA,計算回收率
分別在選取的4份健康小鼠血清、血漿、細胞培養上清中加入高濃度小鼠sIgA,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
Effect of the β-glucan from Lentinus edodes on colitis-associated colorectal cancer and gut microbiota
Rank: IF 10.7
Journal: Carbohydrate Polymers
Year: 2023
DOI: 10.1016/j.carbpol.2023.121069
Self-adjuvant multiepitope nanovaccine based on ferritin induced long-lasting and effective mucosal immunity against H3N2 and H1N1 viruses in mice
Rank: IF 7.7
Journal: International Journal of Biological Macromolecules
Year: 2024
DOI: 10.1016/j.ijbiomac.2024.129259
Polysaccharide from Areca catechu L. inflorescence enhances the intestinal mucosal immunity to maintain immune homeostasis
DOI: 10.1016/j.ijbiomac.2024.134900
Lactobacillus casei ATCC 393 combined with vasoactive intestinal peptidealleviates dextran sodium sulfate-induced ulcerative colitis in C57BL/6mice via NF-κB and Nrf2 signaling pathways
Rank: IF 6.9
Journal: Biomedicine & Pharmacotherapy
DOI: 10.1016/j.biopha.2023.115033
Beneficial effects of Dendrobium officinale National Herbal Drink on metabolic immune crosstalk via regulate SCFAs-Th17/Treg
Rank: IF 6.7
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2024.155816
Dietary supplementation with biogenic selenium nanoparticles alleviate oxidative stress-induced intestinal barrier dysfunction
Rank: IF 6.3
Journal: npj Science of Food
Year: 2022
DOI: 10.1038/s41538-022-00145-3
Prophylactic supplementation with selenium nanoparticles protects against foodborne toxin zearalenone-induced intestinal barrier dysfunction
Rank: IF 6.2
Journal: Ecotoxicology and Environmental Safety
DOI: 10.1016/j.ecoenv.2024.116914
Replacing dietary sodium selenite with biogenic selenium nanoparticles improves the growth performance and gut health of early-weaned piglets
Rank: IF 6.1
Journal: Animal Nutrition
DOI: 10.1016/j.aninu.2023.08.003
Fucoidan-Mediated Covalent Modification Mitigates Allergenicity of Shrimp (Penaeus vannamei) in Mice via Enhanced Intestinal Barrier Function and Antigen Presentation Suppression
Rank: IF 5.7
Journal: Journal of Agricultural and Food Chemistry
DOI: 10.1021/acs.jafc.4c09800
Polygonatum sibiricum Saponin Prevents Immune Dysfunction and Strengthens Intestinal Mucosal Barrier Function in Cyclophosphamide-Induced Immunosuppressed BALB/c Mice
Rank: IF 4.7
Journal: Foods
DOI: 10.3390/foods13060934