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大鼠環磷酸腺苷(cAMP)ELISA檢測試劑盒

 一鍵復制產品信息
英文名稱:Rat Cyclic Adenosine Monophosphate (cAMP) ELISA Kit
實驗類型: 競爭法
檢測范圍: 3.12-200ng/mL
靈敏度: 1.6ng/mL
種屬: 大鼠
保存溫度: 2-8℃
樣本類型: 血清, 血漿, 組織勻漿, 細胞裂解液, 細胞培養上清 和 其他生物液體
產品說明書
品牌 貨號 純度/規格 包裝 市場價 會員價格 庫存 數量 購物車
江萊生物 JL10117-48T 48T 1盒 ¥1980.00
江萊生物 JL10117-96T 96T 1盒 ¥2580.00
江萊生物 JL10117-5×96T 5×96T 1盒 ¥10965.00

|   別稱

 

c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

 

 

|   cAMP 簡介

 

環磷酸腺苷(cAMP)是在許多生物過程中很重要的第二信使。cAMP 是三磷酸腺苷(ATP)的衍生物,在許多不同的生物體中用于細胞內信號轉導,如將胰高血糖素和腎上腺素等不能通過質膜的激素的作用轉移到細胞中。它不應與 5'-AMP 激活的蛋白激酶(AMP-activated protein kinase)相混淆。它還參與了蛋白激酶的激活。此外,cAMP 結合并調節離子通道的功能,如 HCN通道和其他一些環核苷酸結合蛋白,如 Epac1 和 RAPGEF2。

 

 

|   特異性

 

可檢測樣本中大鼠的cAMP,且與其類似物無明顯交叉反應

 

 

|   典型數據及參考曲線

 

由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。

 

濃度(ng/mL) 200 100 50 25 12.5 6.25 3.12 0
OD值 0.216 0.298 0.417 0.669 0.955 1.405 1.79 1.865

 

 

|   重復性

 

板內變異系數小于10%,板間變異系數小于10%

 

 

|   回收率

 

在選取的健康大鼠血清、血漿、細胞培養上清中加入3個不同濃度水平的大鼠cAMP,計算回收率

 

樣本類型 范圍 平均回收率
血清(n=8) 81-101 96
血漿(n=8) 92-105 101
細胞培養上清(n=8) 97-108 105

 

 

|   線性稀釋

 

分別在選取的4份健康大鼠血清、血漿、細胞培養上清中加入高濃度大鼠cAMP,在標準曲線動力學范圍內進行稀釋,評估線性。

 

稀釋比例 回收率(%) 血清 血漿 細胞培養上清
1:2 范圍(%) 83-95 88-96 90-110
平均回收率(%) 91 93 96
1:4 范圍(%) 89-104 87-108 105-115
平均回收率(%) 93 98 110

 

 

注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。

 

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