ICSH; Lutropin; Interstitial Cell Stimulating Hormone
促黃體激素(LH)是由垂體前葉的促性腺激素細胞產生的一種激素。LH的急性上升("LH激增")會觸發排卵和黃體的發育。它也被稱為間質細胞刺激素(ICSH),能刺激Leydig細胞產生睪酮。它有一個120個氨基酸的β亞單位(LHB),賦予其特定的生物作用,并負責與LH受體相互作用的特殊性。這個β亞單位含有一個氨基酸序列,與hCG的β亞單位有很大的同源性,兩者都能刺激相同的受體。
可檢測樣本中大鼠的LH,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
注意:本圖僅供參考,應以同次試驗標準品所繪標準曲線計算標本含量。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康大鼠血清、血漿中加入3個不同濃度水平的大鼠LH,計算回收率
分別在選取的4份健康大鼠血清、血漿中加入高濃度大鼠LH,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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Journal: Biomedicine & Pharmacotherapy
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Year: 2022
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Rank: IF 5.7
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DOI: 10.1021/acs.jafc.4c01151
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Rank: IF 4.8
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DOI: 10.1016/j.jep.2021.114862
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Rank: IF 4.7
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Rank: IF 4.4
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Rank: IF 4
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