CORT
皮質酮,又稱 17-脫氧皮質醇和 11β,21-二羥基黃體酮,是一種 21 碳的皮質 類固醇激素,在腎上腺皮質中產生。除了 17α-羥化酶缺乏導致的非常罕見的 先天性腎上腺增生癥外,它對人類的重要性不大。在許多物種中,包括兩棲 動物、爬行動物、嚙齒動物和鳥類,皮質酮是一種主要的糖皮質激素,參與 調節能量、免疫反應和壓力反應。皮質酮通過醛固酮合成酶轉化為醛固酮, 醛固酮合成酶僅在腎小球細胞的線粒體中發現。腎小球細胞存在于腎小球區, 它是腎上腺皮質內分泌細胞的最表層區域。皮質酮是礦物皮質激素醛固酮的 前體分子,是體內鈉和鉀水平的主要平衡調節劑之一。
可檢測樣本中小鼠的CORT,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿中加入3個不同濃度水平的小鼠CORT,計算回收率
分別在選取的4份健康小鼠血清、血漿中加入高濃度小鼠CORT,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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