本公司產品僅供科研使用,不用于人體及臨床診斷

小鼠雌二醇(E2)ELISA檢測試劑盒

 一鍵復制產品信息
英文名稱: Mouse Estradiol (E2) ELISA kit
實驗類型: 競爭法
檢測范圍: 0.78-50pg/mL
靈敏度:0.29pg/mL
種屬: 小鼠
保存溫度: 2-8℃
樣本類型:血清, 血漿, 組織勻漿, 細胞裂解液, 細胞培養上清 和其他生物液體
產品說明書
品牌 貨號 純度/規格 包裝 市場價 會員價格 庫存 數量 購物車
江萊生物 JL11790-48T 48T 1盒 ¥1800.00
江萊生物 JL11790-96T 96T 1盒 ¥2300.00
江萊生物 JL11790-5×96T 5×96T 1盒 ¥9775.00

|   別稱

 

E2

 

 

|   E2 簡介

 

雌二醇(Estradiol,E2),亦稱“動情素”、“求偶素”。化學式 C18H24O2, 分子量為 272.38,有α,β兩種類型,雌激素的一種。含量最多,活性也最強。由卵巢內卵泡的顆粒細胞分泌,其代謝物是雌酮及雌三醇。含 18 個碳原子。其靶器官為子宮、陰道、輸卵管和垂體。可作為一種為經皮膚吸收的 雌激素治療劑。雌激素能促使細胞合成 DNA、RNA 和相應組織內各種不同的蛋白質。

 

 

|   特異性

 

可檢測樣本中小鼠的E2,且與其類似物無明顯交叉反應

 

 

|   典型數據及參考曲線

 

由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。

 

濃度(pg/mL) 50 25 12.5 6.25 3.12 1.56 0.78 0
OD值 0.188 0.27 0.389 0.642 0.929 1.38 1.766 1.842

 

 

|   重復性

 

板內變異系數小于10%,板間變異系數小于10%

 

 

|   回收率

 

在選取的健康小鼠血清、血漿和細胞培養上清中加入3個不同濃度水平的小鼠E2,計算回收率

 

樣本類型 范圍 平均回收率
血清(n=8) 81-101 96
血漿(n=8) 92-105 101
細胞培養上清(n=8) 97-108 105

 

 

|   線性稀釋

 

分別在選取的4份健康小鼠血清、血漿和細胞培養上清中加入高濃度小鼠E2,在標準曲線動力學范圍內進行稀釋,評估線性。

 

稀釋比例 回收率(%) 血清 血漿 細胞培養上清
1:2 范圍(%) 83-95 88-96 90-110
平均回收率(%) 91 93 96
1:4 范圍(%) 89-104 87-108 105-115
平均回收率(%) 93 98 110

 

 

注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。

 

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