E2
雌二醇(Estradiol,E2),亦稱“動情素”、“求偶素”。化學式 C18H24O2, 分子量為 272.38,有α,β兩種類型,雌激素的一種。含量最多,活性也最強。由卵巢內卵泡的顆粒細胞分泌,其代謝物是雌酮及雌三醇。含 18 個碳原子。其靶器官為子宮、陰道、輸卵管和垂體。可作為一種為經皮膚吸收的 雌激素治療劑。雌激素能促使細胞合成 DNA、RNA 和相應組織內各種不同的蛋白質。
可檢測樣本中小鼠的E2,且與其類似物無明顯交叉反應。
由于實驗操作條件的不同( 如操作者、移液技術、洗板技術和穩定條件等),標準曲線的OD值會有所差異。以下數據和曲線僅供參考,實驗者需根據自己的實驗建立標準曲線。
板內變異系數小于10%,板間變異系數小于10%
在選取的健康小鼠血清、血漿和細胞培養上清中加入3個不同濃度水平的小鼠E2,計算回收率
分別在選取的4份健康小鼠血清、血漿和細胞培養上清中加入高濃度小鼠E2,在標準曲線動力學范圍內進行稀釋,評估線性。
注:ELISA試劑盒檢測范圍等數據,因檢測樣本 的不同而調整,實際數據以隨貨說明書為準。
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