| H3N2
The hemagglutinin (HA) of influenza A virus (H3N2) is a key trimeric glycoprotein on its surface. Acting as the “key” for viral entry, HA specifically recognizes and binds to sialic acid receptors (preferring α -2,6-linked) on the surface of human respiratory cells via its head receptor-binding domain. Subsequently, triggered by the acidic environment of endosomes, HA undergoes an irreversible conformational change, exposing the fusion peptide. This drives fusion between the virus and the host cell membrane, releasing genetic material to initiate infection.
| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
| Concentration (ng/mL) |
30 |
15 |
7.5 |
3.75 |
1.87 |
0.93 |
0.46 |
0 |
| OD |
2.7 |
2.05 |
1.31 |
0.77 |
0.49 |
0.3 |
0.21 |
0.1 |
| Corrected OD |
2.6 |
1.95 |
1.21 |
0.67 |
0.39 |
0.2 |
0.12 |
- |
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
| Recovery
The recovery of H3N2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type |
Range(%) |
Average Recovery(%) |
| Serum(n=8) |
82-105 |
96 |
| Plasma(n=8) |
87-98 |
93 |
| Linearity
Samples were spiked with high concentrations of H3N2 and diluted with Universal Diluent to produce samples with values within the range of the assay.
| |
|
Serum(n=4) |
Plasma(n=4) |
| 1 : 2 |
Range (%) |
100-114 |
86-104 |
| Average (%) |
105 |
97 |
| 1 : 4 |
Range (%) |
82-105 |
96-114 |
| Average (%) |
98 |
103 |
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